AMCA-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH2是组织蛋白酶 D(cathepsin D)(Km = 53 µM, kcat = 7.28 s-1, kcat/Km = 0.14 µM-1s-1)的一个特异性底物，单切割位点位于两个苯丙氨酸(Phe)之间。多肽的N端修饰了AMCA-fluorophore (7-amino-4-methylcoumarin-3-acetic acid)，C端赖氨酸侧链的氨基修饰了biotin。经过酶水解后，biotin-偶联的切割产物和未被水解的底物AMCA-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH2可以用链霉亲和素-包被的磁珠去除。溶液中剩余的荧光多肽代表了被水解的底物的量。

序列(三字母)：AMCA-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH2
序列(单字母)：AMCA-EEKPISFFRLGK(biotinyl)-NH2
别名：(7-Amino-4-methylcoumarin-3-yl)acetyl-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH2
分子式:C90H131N21O22S
分子量:1891.22
CAS#:851231-19-1
合成方法：Synthetic
存储条件：-20 °C，避光，干燥
应用：Biotinylated Peptides